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Exciting opportunity for motivated research technician to join a translational laboratory studying various aspects of cellular adaptive immunity, especially in relation to T regulatory cell characterization, function and expansion, as well as the effects of various immune manipulations. We have a history of successfully conducting basic and preclinical experiments on new biologic agents and translating them into the clinic, inclluding clinical trials studying the administration of T regulatory cells in solid organ transplant settings and in cancer.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

A successful candidate will have the following characteristics:

• Reliable, organized, and flexible with ability to handle multiple activities and to reprioritize tasks

• Understands lab etiquette and participation in general laboratory responsibilities (ordering and organizing laboratory supplies and reagents)

• Perform experiments after usual instruction and supervision

• Ability to analyze information in real-time and use logic to address work-related issues and problems

• Must be attentive to detail and be thorough in completing work tasks

• Able to work with other team members and collaborators as well as complete independent work and show initiative

• Must have excellent communication skills (written and verbal) in order to work in a team environment.

• Able to work under the supervision of PI and staff scientists to analyze data and interpret results of experiments and participate in or take responsibility for generation of lab guidelines and protocols

SUPERVISORY RESPONSIBILITIES:

Research Technician

No supervisory responsibilities. Normally receives detailed instructions on all work.

• Qualifications

• Bachelor’s degree with some experience and desire to learn considered. Coursework in related fields (such as Immunology, Molecular Biology, Quantitative Sciences and Engineering) a plus. Relevant experience in academia or industry strongly preferred.

• Preference given to candidates with adaptive and/or innate immunology experience, including assay development; cell imaging and analysis, isolation and culture of peripheral blood cells for characterization, purification, and cryopreservation; sterile technique; cell-based assays including mixed lymphocyte culture and use of various molecular biology and immunology techniques to assess immune responses. Gene editing experience a plus.

• Other relevant experience includes multianalyte analysis from media or cell lysates (e.g. Luminex), ELISA, DNA and RNA isolation, PCR and RT-PCR, gene editing, various microarray technologies. Assay development and adaptation experience a strong plus.

• Experience with flow cytometry, use of Flow Jo desirable

• Experience in analyzing biological data using GraphPad Prism and Excel required.

• Experience using existing software programs, web-based interactive tools, or database queries as sequence management, array analysis and other analytic needs evolve.

• We are looking for candidates who have at least two years of availability.Highly desirable

• Experience using bioinformatics tools to visualize, processing and analyzing genomic or other molecular data using software packages, statistical applications, or data mining techniques, including use pf publicly available datasets.

• Maintain awareness of new and emerging computational methods and technologies.

• Prior programming experience in scripting languages such as Python, R etc.

• Experience with FACS-based ELISA methods such as LEGENDplex assay and software

• Experience with Adobe Photoshop & Illustrator

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff. Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

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