Joslin Diabetes Center Sr. Research Assistant (Genome Editing Core) in Boston, Massachusetts
Under direct supervision, the Senior Research Assistant will be involved in a DRC-funded core facility for mouse genome editing. Specifically, this individual will be responsible for running all the mouse genome editing activities of the core.
For more information on the laboratory’s focus go to: https://www.joslin.org/find-an-expert/stephan-kissler#
Manage the transgenesis mouse colony, including
Perform vasectomies and maintain cohort of vasectomized males
Order and mate female mice for generation of pseudopregnant foster mice
Order and maintain colony of C57BL/6 and NOD stud male mice
Order, superovulate and mate C57BL/6 and NOD female mice to generate zygotes for microinjection
Perform zygote microinjections
Pronuclear injections for CRISPR/Cas9 genome editing
Perivitelline injections for lentiviral transgenesis
Perform embryo-transfer surgeries
Track pregnancies and genome edited litters
Generate and freeze zygotes to cryopreserve valuable mouse stocks
Track and manage service requests
Communicate with requesting researchers to coordinate schedules and up-date on progress
Advise requesting researcher on available genome editing strategies and methods, with support from core director.
Once genome edited strategy is finalized, customize and order genome editing constructs for each project.
Keep up to date with latest genome editing methods and strategies to implement state-of-the-art methods in the Joslin Core
Coordinate mouse transfers and shipments with requesting researchers inside Joslin and with outside institutions.
Report to supervisor with summary of requests, progress and problems
Optimize workflow and mating efficiency on an on-going basis
Maintain a clean and organized work environment in the microinjection room
Maintain laboratory equipment and inventory.
Assist in the placement of orders for supplies, consumables, and reagents as needed; log delivery of supplies, and store supplies upon delivery.
Perform routine maintenance and cleaning on small equipment as required
Recognize equipment and testing problems, troubleshoot and document independently and/or with assistance of other lab members to ensure the resolution is documented, implemented and communicated in a timely manner
Keep detailed inventory of all current mice
Maintain database with scheduled, ongoing and completed service requests
Perform core facility service billing tasks through iLab
Other duties as assigned
Minimum 1 year of hands-on laboratory experience - observational laboratory work undertaken as part of undergraduate program is insufficient.
Experience with animal handling and husbandry.
Intermediate knowledge of Microsoft Office Suite
Strong verbal and written communication skills
Intermediate analytical, critical thinking, and organizational skills
Strong interpersonal and customer service skills: to interact with other employees, professional staff and/or external contacts
Must be detail oriented, with the ability to prioritize responsibilities – through completion
Ability to work independently and with others in the lab
Basic understanding of molecular cloning, gene editing and mouse genetics.
Requisition Number (add R/N and FY after populates): R3331-21
External Company Name: Joslin Diabetes
External Company URL: http://www.joslin.harvard.edu/
Street: One Joslin Place